Glossary entry (derived from question below)
Portuguese term or phrase:
PCR individualmente para cada par de primer
English translation:
each primer pair was individually submitted to PCR.
Added to glossary by
Christina Paiva
Apr 11, 2011 20:50
13 yrs ago
2 viewers *
Portuguese term
Brazil
Genuine dedication and professionalism
Native in: Portuguese (Variant: Brazilian) 
Works in: English to Portuguese, Spanish to Portuguese
PCR individualmente para cada par de primer
Portuguese to English
Other
Genetics
O DNA genômico foi extraído a partir de 3 x 106 células utilizando o kit DNAzol (Invitrogen®), conforme protocolo proposto pelo fabricante, seguido pela PCR individualmente para cada par de primer aroA e dpr utilizando 200 µM dNTP, 3,4 mM MgCl2, 2 U de Taq DNA Polimerase (Invitrogen®), 1,6x tampão de amplificação e 0,20 μM dos primers em um volume final de 25 µL.
Proposed translations
(English)
| 4 | each primer pair was individually submitted to PCR. |
Christina Paiva
|
| 4 | followed by PCR individually for each primer pair |
Walter Moura
|
Change log
Apr 18, 2011 14:13: Christina Paiva Created KOG entry
Proposed translations
8 hrs
Christina Paiva
Brazil
Brazil
Native in Portuguese
&
English
Specializes in field
Brazil
Science-driven Sworn Translations
Native in: Portuguese (Variant: Brazilian) 
, English (Variant: US)
Works in: Portuguese to English, English to Portuguese
Related KudoZ points
:
12
Selected
each primer pair was individually submitted to PCR.
Sugestão:
by the manufacturer. Subsequently, each...
by the manufacturer. Subsequently, each...
4 KudoZ points awarded for this answer.
3 hrs
Walter Moura
Brazil
Brazil
Native in Portuguese
Brazil
Your message. Clearly and on time.
Native in: Portuguese (Variant: Brazilian)
Works in: English to Portuguese
Related KudoZ points
:
6
followed by PCR individually for each primer pair
http://en.wikipedia.org/wiki/Polymerase_chain_reaction
The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
Multiplex Ligation-dependent Probe Amplification (MLPA): permits multiple targets to be amplified with only a single primer pair, thus avoiding the resolution limitations of multiplex PCR (see below).
http://www.med.nyu.edu/rcr/rcr/course/primer.html
*** For PCR primer pair selection, you can choose a target range of the template sequence to be amplified.
The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
Multiplex Ligation-dependent Probe Amplification (MLPA): permits multiple targets to be amplified with only a single primer pair, thus avoiding the resolution limitations of multiplex PCR (see below).
http://www.med.nyu.edu/rcr/rcr/course/primer.html
*** For PCR primer pair selection, you can choose a target range of the template sequence to be amplified.
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